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Home > Product > ELISA Kit > Canine Apolipoprotein E, APOE ELISA Kit

Canine Apolipoprotein E, APOE ELISA Kit

Canine Apolipoprotein E, APOE ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 9.91ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 9.91ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Apolipoprotein E

Code

E0329Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

9.91ng/mL

Detection range

20-4500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.

UniProt accession

MASS(Da)

37,224

GeneID

476438

Synonyms

APOE;Apo-E;Apolipoprotein E

Gene names

APOE

Research area

Signal Transduction; Cardiovascular

Target protein

APOE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0329Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/mL 2.148 2.225 2.162
2.303
1200ng/mL 1.553 1.447 1.383
1.34
600ng/mL 0.855 0.831 0.768
0.807
300ng/mL 0.536 0.536 0.473
0.537
150ng/mL 0.217 0.241 0.178
0.265
0ng/mL 0.063 0.063 0
0.063

Precision

We measured random samples of E0329Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1318 44.57 3.4
Intra-Assay 2 18 385.8 23.39 6.1
Intra-Assay 3 18 548.5 31.53 5.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0329Ca

    $475.00

  • 48T

    E0329Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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