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Home > Product > ELISA Kit > Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Human DNA Replication Factor Cdt1, CDT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.63ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dna Replication Factor Cdt1

Code

E5191Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.63ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for both DNA replication and mitosis (PubMed: 11125146, PubMed: 22581055, PubMed: 21856198, PubMed: 14993212, PubMed: 26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed: 14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed: 22581055). Potential oncogene (By similarity).

UniProt accession

MASS(Da)

60,390

GeneID

81620

Synonyms

CDT 1;CDT1;DNA replication factor Cdt1;Double parked homolog;DUP

Gene names

CDT1

Research area

Epigenetics and Nuclear Signaling

Target protein

CDT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human DNA Replication Factor Cdt1, CDT1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.686 2.566 2.517
2.446
800ng/L 1.581 1.492 1.443
1.403
400ng/L 0.92 0.925 0.876
0.93
200ng/L 0.413 0.46 0.411
0.506
100ng/L 0.249 0.218 0.169
0.188
0ng/L 0.041 0.049 0
0.057

Precision

We measured random samples of E5191Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 668.2 22.18 3.3
Intra-Assay 2 18 464.4 23.9 5.1
Intra-Assay 3 18 181.4 10.7 5.9

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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