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Home > Product > ELISA Kit > Human Fibroblast Growth Factor Receptor 3, FGFR3 ELISA Kit

Human Fibroblast Growth Factor Receptor 3, FGFR3 ELISA Kit

Human Fibroblast Growth Factor Receptor 3, FGFR3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.93ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 14.93ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fibroblast Growth Factor Receptor 3

Code

E5290Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.93ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.

UniProt accession

MASS(Da)

87,710

GeneID

2261

Synonyms

CD antigen CD333;FGFR 3;FGFR3;FGFR-3;Fibroblast growth factor receptor 3

Gene names

FGFR3

Research area

Signal Transduction

Target protein

FGFR3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5290Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.051 2.103 2.064
2.154
2400ng/L 1.384 1.424 1.385
1.464
1200ng/L 0.775 0.664 0.626
0.554
600ng/L 0.389 0.385 0.346
0.381
300ng/L 0.138 0.162 0.123
0.186
0ng/L 0.03 0.039 0
0.047

Precision

We measured random samples of E5290Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1834 76.97 4.2
Intra-Assay 2 18 2596.7 97.64 3.8
Intra-Assay 3 18 813.6 36.1 4.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5290Hu

    $320.00

  • 96T

    E5290Hu

    $458.00

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Lead time: Within two weeks

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