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Home > Product > ELISA Kit > Human Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Human Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Human Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.38μU/mL

Detection range: 5-1000uU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.38μU/mL

Detection range: 5-1000uU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glutamate Dehydrogenase 1, Mitochondrial

Code

E5335Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.38μU/mL

Detection range

5-1000uU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed: 11032875, PubMed: 16959573, PubMed: 11254391, PubMed: 16023112). Plays a role in insulin homeostasis (PubMed: 9571255, PubMed: 11297618). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

UniProt accession

MASS(Da)

61,398

GeneID

2746

Synonyms

GDH 1;GLUD 1;GLUD1;Glutamate dehydrogenase 1, mitochondrial

Gene names

GLUD1

Research area

Signal Transduction

Target protein

GLUD1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5335Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640μU/mL 2.046 2.167 2.121
2.288
320μU/mL 1.202 1.189 1.143
1.176
160μU/mL 0.855 0.77 0.724
0.684
80μU/mL 0.442 0.426 0.381
0.411
40μU/mL 0.179 0.205 0.16
0.231
0μU/mL 0.054 0.046 0
0.037

Precision

We measured random samples of E5335Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 274.9 17.08 6.2
Intra-Assay 2 18 496 16.21 3.3
Intra-Assay 3 18 116.4 6.89 5.9

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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