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Home > Product > ELISA Kit > Human Interferon-induced 35 kDa Protein, IFI35 ELISA Kit

Human Interferon-induced 35 kDa Protein, IFI35 ELISA Kit

Human Interferon-induced 35 kDa Protein, IFI35 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.28ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.28ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interferon-Induced 35 Kda Protein

Code

E5498Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.28ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a signaling pathway regulator involved in innate immune system response (PubMed: 26342464, PubMed: 29038465, PubMed: 29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed: 10779520, PubMed: 10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed: 26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed: 29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed: 29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed: 29038465).

UniProt accession

MASS(Da)

31,546

GeneID

3430

Synonyms

IFI 35;IFI35;Ifi-35;IFP 35;Interferon-induced 35 kDa protein

Gene names

IFI35

Research area

Cell Biology

Target protein

IFI35

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5498Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.051 2.065 2.022
2.078
800ng/L 1.845 1.878 1.836
1.912
400ng/L 1.251 1.178 1.135
1.104
200ng/L 0.753 0.743 0.7
0.732
100ng/L 0.397 0.413 0.371
0.429
0ng/L 0.047 0.042 0
0.038

Precision

We measured random samples of E5498Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 437.8 22.76 5.2
Intra-Assay 2 18 156.5 7.92 5.1
Intra-Assay 3 18 240.3 10.85 4.5

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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