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Home > Product > ELISA Kit > Chicken Interleukin 6, IL-6 ELISA Kit

Chicken Interleukin 6, IL-6 ELISA Kit

Chicken Interleukin 6, IL-6 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Chicken

Product Summary

Size: 96T,48T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Chicken

Product overview

Full product name

Interleukin 6

Code

E0004Ch

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.53ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Chicken

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.

UniProt accession

MASS(Da)

26,790

GeneID

395337

Synonyms

ChIL-6;IL 6;IL6;IL-6;Interleukin-6

Gene names

IL6

Research area

Immunology; Neuroscience; Microbiology; Cancer; Metabolism

Target protein

IL6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0004Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.327 2.275 2.231
2.223
120ng/L 1.257 1.208 1.164
1.159
60ng/L 0.624 0.661 0.617
0.698
30ng/L 0.53 0.518 0.474
0.505
15ng/L 0.183 0.188 0.145
0.193
0ng/L 0.062 0.044 0
0.025

Precision

We measured random samples of E0004Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 182.2 5.4 3
Intra-Assay 2 18 26 1.41 5.4
Intra-Assay 3 18 110.3 5.35 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0004Ch

    $498.00

  • 48T

    E0004Ch

    $360.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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