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Home > Product > ELISA Kit > Human Protein-tyrosine Kinase 2-beta, PTK2B ELISA Kit

Human Protein-tyrosine Kinase 2-beta, PTK2B ELISA Kit

Human Protein-tyrosine Kinase 2-beta, PTK2B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.37ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.37ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein-Tyrosine Kinase 2-Beta

Code

E6025Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.37ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion Channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium Channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

UniProt accession

MASS(Da)

115,875

GeneID

2185

Synonyms

CADTK;CAKB;CAK-beta;Calcium-dependent tyrosine kinase;Calcium-regulated non-receptor proline-rich tyrosine kinase;Cell adhesion kinase beta;FADK 2;Focal adhesion kinase 2;Proline-rich tyrosine kinase 2;Protein-tyrosine kinase 2-beta;PTK 2B;PTK2B;RAFTK;Related adhesion focal tyrosine kinase

Gene names

PTK2B

Research area

Others

Target protein

PTK2B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6025Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.285 2.241 2.202
2.197
800ng/L 1.563 1.606 1.567
1.649
400ng/L 1.196 1.112 1.073
1.027
200ng/L 0.643 0.637 0.598
0.631
100ng/L 0.473 0.46 0.421
0.448
0ng/L 0.046 0.039 0
0.032

Precision

We measured random samples of E6025Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 585.9 26.06 4.4
Intra-Assay 2 18 248.9 16.5 6.6
Intra-Assay 3 18 87.9 6.56 7.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6025Hu

    $320.00

  • 96T

    E6025Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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