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Home > Product > ELISA Kit > Human Interleukin-10 Receptor Subunit Alpha, IL10RA ELISA Kit

Human Interleukin-10 Receptor Subunit Alpha, IL10RA ELISA Kit

Human Interleukin-10 Receptor Subunit Alpha, IL10RA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-10 Receptor Subunit Alpha

Code

E6471Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed: 16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed: 12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed: 26962683).

UniProt accession

MASS(Da)

63,003

GeneID

3587

Synonyms

CD antigen CD210;CDw210a;IL 10RA;IL-10 receptor subunit alpha;IL-10R subunit 1;IL-10R subunit alpha;IL-10R1;IL10RA;IL-10RA;Interleukin-10 receptor subunit 1;Interleukin-10 receptor subunit alpha

Gene names

IL10RA

Research area

Immunology

Target protein

IL10RA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Interleukin-10 Receptor Subunit Alpha, IL10RA ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.418 2.32 2.266
2.223
6ng/ml 1.288 1.314 1.26
1.34
3ng/ml 0.789 0.831 0.776
0.872
1.5ng/ml 0.432 0.422 0.367
0.411
0.75ng/ml 0.31 0.279 0.225
0.249
0ng/ml 0.048 0.054 0
0.06

Precision

We measured random samples of E6471Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.5 0.24 4.5
Intra-Assay 2 18 6.9 0.24 3.5
Intra-Assay 3 18 2 0.14 7

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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