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Horse Interleukin 10, IL10 ELISA Kit

Horse Interleukin 10, IL10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Interleukin 10

Code

E0128HO

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.45ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

MASS(Da)

20,451

GeneID

100034187

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Horse Interleukin 10, IL10 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.79 1.86 1.816
1.93
600ng/L 0.906 0.958 0.915
1.011
300ng/L 0.461 0.469 0.425
0.477
150ng/L 0.296 0.229 0.186
0.163
75ng/L 0.112 0.085 0.041
0.059
0ng/L 0.047 0.044 0
0.041

Precision

We measured random samples of E0128HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 228.7 7.55 3.3
Intra-Assay 2 18 836.2 26.74 3.2
Intra-Assay 3 18 721.8 42.24 5.9

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $360.00

  • 96T

    $498.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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