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Home > Product > ELISA Kit > Mouse Interleukin 10, IL-10 ELISA Kit

Mouse Interleukin 10, IL-10 ELISA Kit

Mouse Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.48pg/ml

Detection range: 5-2000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 2.48pg/ml

Detection range: 5-2000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin 10

Code

E0022Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.48pg/ml

Detection range

5-2000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed: 28473584).

UniProt accession

MASS(Da)

20,641

GeneID

16153

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Interleukin 10, IL-10 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200pg/ml 2.143 2.238 2.169
2.333
600pg/ml 1.25 1.241 1.172
1.232
300pg/ml 0.817 0.778 0.708
0.739
150pg/ml 0.521 0.462 0.393
0.404
75pg/ml 0.312 0.308 0.238
0.303
0pg/ml 0.076 0.069 0
0.063

Precision

We measured random samples of E0022Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 533.3 30.76 5.8
Intra-Assay 2 18 673.7 21.35 3.2
Intra-Assay 3 18 210 12.39 5.9

Protocols

References

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice
Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice
Hyperbaric Oxygen Effect on Switching Phenotype M1-M2 Macrophage Expression in Antigen and Collagen-Induced Arthritis.

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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