Product overview
Full product name
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Background
Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed: 28953886, PubMed: 28846097, PubMed: 28846098, PubMed: 28846099, PubMed: 23468844, PubMed: 29046435). Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed: 28953886, PubMed: 28846097, PubMed: 28846098, PubMed: 28846099). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed: 28953886). On hepatocytes, inhibits growth hormone signaling (By similarity).
UniProt accession
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Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0037Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1600ng/L | 2.076 | 2.016 | 1.983 |
1.956 | |||
800ng/L | 1.151 | 1.157 | 1.125 |
1.163 | |||
400ng/L | 0.561 | 0.573 | 0.54 |
0.585 | |||
200ng/L | 0.271 | 0.282 | 0.25 |
0.294 | |||
100ng/L | 0.072 | 0.121 | 0.089 |
0.17 | |||
0ng/L | 0.033 | 0.032 | 0 |
0.032 |
Precision
We measured random samples of E0037Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 248.1 | 9.75 | 3.9 |
Intra-Assay | 2 | 18 | 1244.8 | 39.23 | 3.2 |
Intra-Assay | 3 | 18 | 603 | 28.98 | 4.8 |
Protocols
Customer reviews and Q&As