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Home > Product > ELISA Kit > Mouse Interleukin 1Β, IL-1B ELISA Kit

Mouse Interleukin 1Β, IL-1B ELISA Kit

Mouse Interleukin 1Β, IL-1B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.12ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.12ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin 1beta

Code

E0192Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.12ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

UniProt accession

MASS(Da)

30,931

GeneID

16176

Synonyms

IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Gene names

IL1B

Research area

Immunology; Microbiology; Cardiovascular; Metabolism

Target protein

IL1B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0192Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.053 2.062 2.015
2.072
1200ng/L 0.988 1.041 0.993
1.094
600ng/L 0.568 0.524 0.476
0.48
300ng/L 0.182 0.179 0.132
0.176
150ng/L 0.165 0.13 0.082
0.094
0ng/L 0.063 0.048 0
0.033

Precision

We measured random samples of E0192Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1080.1 42.14 3.9
Intra-Assay 2 18 1671.5 46.56 2.8
Intra-Assay 3 18 668 34.16 5.1

Protocols

References (2)

Zingerone Alleviates Morphine Tolerance and Dependence in Mice by Reducing Oxidative Stress-Mediated NLRP3 Inflammasome Activation
Trimetazidine attenuates cyclophosphamide-induced cystitis by inhibiting TLR4-mediated NFκB signaling in mice

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  • Size

    Code

    Price

  • 48T

    E0192Mo

    $320.00

  • 96T

    E0192Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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