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Home > Product > ELISA Kit > Human Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Human Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Human Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Vascular Endothelial Cell Growth Factor A

Code

E0050Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.52ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

UniProt accession

MASS(Da)

27,042

GeneID

7422

Synonyms

Vascular endothelial growth factor A;Vascular permeability factor;VEGF A;VEGFA;VEGF-A;VPF

Gene names

VEGFA

Research area

Cancer

Target protein

VEGFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.628 2.533 2.472
2.438
240ng/L 1.444 1.451 1.391
1.458
120ng/L 0.825 0.771 0.711
0.718
60ng/L 0.51 0.495 0.435
0.48
30ng/L 0.246 0.254 0.193
0.262
0ng/L 0.065 0.06 0
0.056

Precision

We measured random samples of E0050Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 61.8 3.38 5.5
Intra-Assay 2 18 176.2 9.42 5.3
Intra-Assay 3 18 208.3 5.53 2.7

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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