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Home > Product > ELISA Kit > Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tumor Necrosis Factor Αlpha

Code

E0082Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.52ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed: 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed: 22517918, PubMed: 16829952, PubMed: 23396208).

UniProt accession

MASS(Da)

25,644

GeneID

7124

Synonyms

APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; tnf alpha; tnf a; tnfa; TNFA_HUMAN; TNFSF2; Tumor necrosis factor; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form

Gene names

TNFA

Research area

Immunology

Target protein

TNFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.949 1.985 1.942
2.02
240ng/L 0.914 1.026 0.983
1.138
120ng/L 0.43 0.515 0.473
0.601
60ng/L 0.293 0.277 0.234
0.261
30ng/L 0.141 0.1 0.057
0.059
0ng/L 0.054 0.043 0
0.032

Precision

We measured random samples of E0082Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 107.9 4.86 4.5
Intra-Assay 2 18 317.7 8.07 2.5
Intra-Assay 3 18 224.5 11.33 5

Protocols

References

Effect of intermittent fasting during Ramadan on Visfatin, Adiponectin and tumor necrotizing factor-alpha in healthy Muslim individuals
Effect of high amylose resistant starch (HAM‐RS2) supplementation on biomarkers of inflammation and oxidative stress in hemodialysis patients: a randomized clinical trial
THE ROLE OF TNF-α AND IL-6 CYTOKINE IN CHILDREN WITH STATUS EPILEPTICUS
Effect of Six Months of Aerobic Exercise on Plasma Interleukin-6 and Tumor Necrosis Factor-Alpha as Breast Cancer Risk Factors in Postmenopausal Women: A Randomized Controlled Trial
Effects of Oral Supplementation With Omega-3 Fatty Acids on Nutritional State and Inflammatory Markers in Maintenance Hemodialysis Patients
The effect of omega-3 fatty acids on depressive symptoms and inflammatory markers in maintenance hemodialysis patients: a randomized, placebo-controlled clinical trial
Effects of Olive Leaf Extract on Metabolic Response, Liver and Kidney Functions and Inflammatory Biomarkers in Hypertensive Patients
TROMBOSIT TERENDAH, KENAIKAN HEMATOKRIT DAN KADAR TNF-α SEBAGAI INDIKASI KEPARAHAN INFEKSI VIRUS DENGUE PADA PASIEN DD DAN DBD
Effects of Olive Leaf Extract on Metabolic Response, Liver and Kidney Functions and Inflammatory Biomarkers in Hypertensive Patients
The role of follicular fluid soluble Receptor for Advanced Glycation End products (sRAGE) on ICSI outcome in PCOS patients.
Nephroprotective effect of Sphaeranthus amaranthoides Burm f.: Sivakaranthai (a Siddha Kayakalpa drug) against gentamicin induced nephrotoxicity
Correlation of Sympathovagal Imbalance with Disease Activity and Inflammatory Markers in South Indian Patients with Rheumatoid Arthritis

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

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Lead time: Within one week

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