Product overview
Full product name
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Assay type
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Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed: 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed: 22517918, PubMed: 16829952, PubMed: 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed: 12794819). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
UniProt accession
MASS（Da）
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0082Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 1.949 | 1.985 | 1.942 |
| 2.02 | |||
| 240ng/L | 0.914 | 1.026 | 0.983 |
| 1.138 | |||
| 120ng/L | 0.43 | 0.515 | 0.473 |
| 0.601 | |||
| 60ng/L | 0.293 | 0.277 | 0.234 |
| 0.261 | |||
| 30ng/L | 0.141 | 0.1 | 0.057 |
| 0.059 | |||
| 0ng/L | 0.054 | 0.043 | 0 |
| 0.032 |
Precision
We measured random samples of E0082Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 107.9 | 4.86 | 4.5 |
| Intra-Assay | 2 | 18 | 317.7 | 8.07 | 2.5 |
| Intra-Assay | 3 | 18 | 224.5 | 11.33 | 5 |
Protocols
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