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Home > Product > ELISA Kit > Human Interleukin 8, IL-8 ELISA Kit

Human Interleukin 8, IL-8 ELISA Kit

Human Interleukin 8, IL-8 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 8

Code

E0089Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.51ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

UniProt accession

MASS(Da)

11,098

GeneID

3576

Synonyms

(Ala-IL-877;(Ser-IL-872;Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;Emoctakin;GCP/IL-8 protein I;GCP/IL-8 protein II;GCP/IL-8 protein III;GCP/IL-8 protein IV;GCP/IL-8 protein V;GCP/IL-8 protein VI;GCP-1;Granulocyte chemotactic protein 1;IL 8;IL8;IL-8;IL-8(1-77;IL-8(5-77;IL-8(6-77;IL-8(7-77;IL-8(8-77;IL-8(9-77;IL8/NAP1 form I;IL8/NAP1 form II;IL8/NAP1 form III;IL8/NAP1 form IV;IL8/NAP1 form V;IL8/NAP1 form VI;Interleukin-8;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF;MDNCF-a;MDNCF-b;MDNCF-c;MONAP;Monocyte-derived neutrophil chemotactic factor;NAF;NAP-1;Monocyte-derived neutrophil-activating Peptide;Neutrophil-activating factor;Neutrophil-activating protein 1;Protein 3-10C;T-cell chemotactic factor;

Gene names

IL8

Research area

Cardiovascular; Immunology; Cancer

Target protein

IL8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0089Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.307 2.349 2.287
2.39
320ng/L 1.251 1.321 1.26
1.391
160ng/L 0.686 0.682 0.62
0.678
80ng/L 0.4 0.402 0.341
0.404
40ng/L 0.318 0.31 0.249
0.303
0ng/L 0.045 0.061 0
0.077

Precision

We measured random samples of E0089Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 134.2 8.57 6.4
Intra-Assay 2 18 276.4 16.51 6
Intra-Assay 3 18 400.5 12.96 3.2

Protocols

References (10)

Comparison of Two Different Inhalation Anesthetics on Grafted Kidney Function in Patients Undergoing Renal Transplantation Surgery: Desflurane or Sevoflurane?
Effects of Olive Leaf Extract on Metabolic Response, Liver and Kidney Functions and Inflammatory Biomarkers in Hypertensive Patients
Effect of IL-6, IL-8/CXCL8, IP-10/CXCL 10 levels on the severity in COVID 19 infection
Evaluation of periodontal status and cytokine response in children with familial Mediterranean fever or systemic juvenile idiopathic arthritis
A Single Vitamin D3 Bolus Supplementation Improves Vitamin D Status and Reduces Proinflammatory Cytokines in Healthy Females
View all publications for the product

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  • Size

    Code

    Price

  • 96T

    E0089Hu

    $458.00

  • 48T

    E0089Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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