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Home > Product > ELISA Kit > Mouse Elav-like Protein 1, ELAVL1 ELISA Kit

Mouse Elav-like Protein 1, ELAVL1 ELISA Kit

Mouse Elav-like Protein 1, ELAVL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Elav-Like Protein 1

Code

E2047Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.75ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (PubMed: 24394384). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (By similarity). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (PubMed: 21402775). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed: 27616329).

UniProt accession

MASS(Da)

36,169

GeneID

15568

Synonyms

ELAVL 1;ELAVL1;Elav-like generic protein;ELAV-like protein 1;Hu-antigen R;HuR;MelG

Gene names

ELAVL1

Research area

Others

Target protein

ELAVL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2047Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.058 2.102 2.059
2.147
800ng/L 1.96 1.876 1.832
1.791
400ng/L 1.189 1.205 1.162
1.221
200ng/L 0.634 0.617 0.573
0.599
100ng/L 0.357 0.353 0.309
0.348
0ng/L 0.054 0.043 0
0.033

Precision

We measured random samples of E2047Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 320 21.05 6.6
Intra-Assay 2 18 447.3 16.09 3.6
Intra-Assay 3 18 220.1 10.38 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2047Mo

    $320.00

  • 96T

    E2047Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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