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Home > Product > ELISA Kit > Mouse Glutamate Dehydrogenase GLDH ELISA Kit

Mouse Glutamate Dehydrogenase GLDH ELISA Kit

Mouse Glutamate Dehydrogenase GLDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 24.35μU/mL

Detection range: 50-15000uU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 24.35μU/mL

Detection range: 50-15000uU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Glutamate Dehydrogenase

Code

E2104Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

24.35μU/mL

Detection range

50-15000uU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress (By similarity).

UniProt accession

MASS(Da)

44813

GeneID

544015

Synonyms

GDH1;GLDH;Glutamate dehydrogenase

Gene names

GDH1

Research area

Others

Target protein

GDH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2104Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000μU/mL 2.115 2.067 2.021
2.02
4000μU/mL 1.521 1.442 1.396
1.364
2000μU/mL 0.56 0.67 0.623
0.78
1000μU/mL 0.371 0.411 0.364
0.452
500μU/mL 0.147 0.151 0.104
0.155
0μU/mL 0.038 0.047 0
0.055

Precision

We measured random samples of E2104Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3181.1 171.34 5.4
Intra-Assay 2 18 3818.7 129.88 3.4
Intra-Assay 3 18 1307.2 56.59 4.3

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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