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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E3300Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.216	2.191	2.159
	2.165
6ng/ml	1.752	1.79	1.758
	1.828
3ng/ml	1.186	1.187	1.155
	1.187
1.5ng/ml	0.618	0.627	0.595
	0.636
0.75ng/ml	0.356	0.385	0.353
	0.414
0ng/ml	0.036	0.032	0
	0.028

We measured random samples of E3300Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1.3	0.1	6.1
Intra-assay	2	18	4.7	0.3	5.6
Intra-assay	3	18	2.5	0.1	5.4

> Protocol Booklet

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