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Home > Product > ELISA Kit > Human Interleukin 10, IL-10 ELISA Kit

Human Interleukin 10, IL-10 ELISA Kit

Human Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.59pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 2.59pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Human

Product overview

Full product name

Interleukin 10

Code

E0102Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.59pg/ml

Detection range

5-1500pg/ml

Sample type

Serum, plasma, cell culture supernates, ascites, tissue homogenates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed: 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed: 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed: 1940799, PubMed: 7512027, PubMed: 11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed: 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

MASS(Da)

20,517

GeneID

3586

Synonyms

Interleukin-10; IL-10; IL10; IL 10; Cytokine synthesis inhibitory factor; CSIF

Gene names

IL10

Research area

Immunology

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Interleukin 10, IL-10 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800pg/ml 2.209 2.312 2.25
2.415
400pg/ml 1.421 1.313 1.251
1.205
200pg/ml 0.706 0.799 0.738
0.892
100pg/ml 0.496 0.45 0.388
0.404
50pg/ml 0.325 0.281 0.22
0.238
0pg/ml 0.058 0.062 0
0.065

Precision

We measured random samples of E0102Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 168.6 9.51 5.6
Intra-Assay 2 18 538.9 12.14 2.3
Intra-Assay 3 18 361.5 20.86 5.8

Protocols

References

Evaluation of Gene Expression Pattern of IL1B and IL10 Cytokines Following Vitamin C Administration among Brain-Dead Liver Donors
Effects of Oral Supplementation With Omega-3 Fatty Acids on Nutritional State and Inflammatory Markers in Maintenance Hemodialysis Patients
The effect of omega-3 fatty acids on depressive symptoms and inflammatory markers in maintenance hemodialysis patients: a randomized, placebo-controlled clinical trial
Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

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Lead time: Within one week

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