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Home > Product > ELISA Kit > Human Interleukin 10, IL-10 ELISA Kit

Human Interleukin 10, IL-10 ELISA Kit

Human Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.59pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 2.59pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 10

Code

E0102Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.59pg/ml

Detection range

5-1500pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed: 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed: 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed: 1940799, PubMed: 7512027, PubMed: 11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed: 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

MASS(Da)

20,517

GeneID

3586

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0102Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800pg/ml 2.209 2.312 2.25
2.415
400pg/ml 1.421 1.313 1.251
1.205
200pg/ml 0.706 0.799 0.738
0.892
100pg/ml 0.496 0.45 0.388
0.404
50pg/ml 0.325 0.281 0.22
0.238
0pg/ml 0.058 0.062 0
0.065

Precision

We measured random samples of E0102Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 168.6 9.51 5.6
Intra-Assay 2 18 538.9 12.14 2.3
Intra-Assay 3 18 361.5 20.86 5.8

Protocols

References (11)

Potential effects of omega-3 fatty acids on anemia and inflammatory markers in maintenance hemodialysis patients
Effects of Oral Supplementation With Omega-3 Fatty Acids on Nutritional State and Inflammatory Markers in Maintenance Hemodialysis Patients
The effect of omega-3 fatty acids on depressive symptoms and inflammatory markers in maintenance hemodialysis patients: a randomized, placebo-controlled clinical trial
The Relationship between Serum Concentrations of Pro- and Anti-Inflammatory Cytokines and Nutritional Status in Patients with Traumatic Head Injury in the Intensive Care Unit
Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors
View all publications for the product

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  • Size

    Code

    Price

  • 96T

    E0102Hu

    $458.00

  • 48T

    E0102Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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