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Home > Product > ELISA Kit > Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.058ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.058ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Insulin-like Growth Factors 1

Code

E0103Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.058ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The Insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed: 21076856, PubMed: 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed: 19578119, PubMed: 22351760, PubMed: 23696648, PubMed: 23243309).

UniProt accession

MASS(Da)

21,841

GeneID

3479

Synonyms

IGF 1;IGF1;IGF-I;Insulin-like growth factor I;Mechano growth factor;MGF;Somatomedin-C

Gene names

IGF1

Research area

Signal Transduction; Cancer; Developmental Biology

Target protein

IGF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0103Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.247 2.19 2.132
2.133
12ng/ml 1.594 1.627 1.569
1.66
6ng/ml 1.023 1.077 1.018
1.13
3ng/ml 0.701 0.674 0.615
0.646
1.5ng/ml 0.443 0.429 0.371
0.415
0ng/ml 0.059 0.058 0
0.058

Precision

We measured random samples of E0103Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.5 0.43 5.1
Intra-Assay 2 18 6.9 0.51 7.4
Intra-Assay 3 18 2.2 0.15 6.7

Protocols

References (3)

Impact of maternal dietary carbohydrate intake and vitamin D-related genetic risk score on birth length: the Vitamin D Pregnant Mother (VDPM) cohort study
Evaluation of the relationship between pain inflammation due to dental caries and growth parameters in preschool children
Evaluation of circulating insulin-like growth factor-1, heart-type fatty acid-binding protein, and endotrophin levels as prognostic markers of COVID-19 infection severity

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  • Size

    Code

    Price

  • 96T

    E0103Hu

    $458.00

  • 48T

    E0103Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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