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Home > Product > ELISA Kit > Mouse Nucleobindin-2, NUCB2 ELISA Kit

Mouse Nucleobindin-2, NUCB2 ELISA Kit

Mouse Nucleobindin-2, NUCB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.9ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.9ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nucleobindin-2

Code

E2315Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.9ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-binding protein which may have a role in calcium homeostasis (PubMed: 10915798). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity). [Nesfatin-1]: Anorexigenic Peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

UniProt accession

MASS(Da)

50,304

GeneID

53322

Synonyms

DNA-binding protein NEFA;Nesfatin-1;NUCB 2;NUCB2;Nucleobindin-2;Prepronesfatin

Gene names

NUCB2

Research area

Others

Target protein

NUCB2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2315Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.864 1.992 1.932
2.12
600ng/L 0.998 1.049 0.989
1.1
300ng/L 0.557 0.575 0.515
0.592
150ng/L 0.318 0.241 0.181
0.165
75ng/L 0.081 0.107 0.047
0.133
0ng/L 0.063 0.06 0
0.057

Precision

We measured random samples of E2315Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 479.4 14.76 3.1
Intra-Assay 2 18 902.4 22.48 2.5
Intra-Assay 3 18 366.7 14.85 4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2315Mo

    $320.00

  • 96T

    E2315Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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