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Home > Product > ELISA Kit > Mouse Transcription Factor Sp1, SP1 ELISA Kit

Mouse Transcription Factor Sp1, SP1 ELISA Kit

Mouse Transcription Factor Sp1, SP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.69ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.69ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Transcription Factor Sp1

Code

E2519Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.69ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity). Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed: 24030830). Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed: 27918959).

UniProt accession

MASS(Da)

80,732

GeneID

20683

Synonyms

SP 1;SP1;Specificity protein 1;Transcription factor Sp1

Gene names

SP1

Research area

Others

Target protein

SP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2519Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.387 2.453 2.39
2.518
1600ng/L 1.471 1.513 1.45
1.555
800ng/L 0.906 0.9 0.837
0.895
400ng/L 0.375 0.431 0.368
0.488
200ng/L 0.222 0.235 0.171
0.247
0ng/L 0.065 0.063 0
0.061

Precision

We measured random samples of E2519Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 372 17.72 4.8
Intra-Assay 2 18 2024 72.89 3.6
Intra-Assay 3 18 889.4 51.24 5.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2519Mo

    $320.00

  • 96T

    E2519Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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