Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Interleukin 18, IL-18 ELISA Kit

Human Interleukin 18, IL-18 ELISA Kit

Human Interleukin 18, IL-18 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.2ng/L

Detection range: 0.5-100ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.2ng/L

Detection range: 0.5-100ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 18

Code

E0147Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.2ng/L

Detection range

0.5-100ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed: 14528293, PubMed: 25500532). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable) (PubMed: 10653850).

UniProt accession

MASS(Da)

22,326

GeneID

3606

Synonyms

Iboctadekin;IFN-gamma-inducing factor;IL 18;IL-1 gamma;IL18;IL-18;Interferon gamma-inducing factor;Interleukin-1 gamma;Interleukin-18;

Gene names

IL18

Research area

Immunology

Target protein

IL18

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0147Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/L 2.016 2.103 2.064
2.19
32ng/L 1.301 1.358 1.319
1.415
16ng/L 0.723 0.651 0.612
0.579
8ng/L 0.421 0.386 0.347
0.351
4ng/L 0.147 0.157 0.118
0.167
0ng/L 0.031 0.039 0
0.046

Precision

We measured random samples of E0147Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 41.4 1.67 4
Intra-Assay 2 18 24.4 1.06 4.3
Intra-Assay 3 18 8.5 0.38 4.5

Protocols

References (10)

Apolipoprotein A2? 265 T> C polymorphism interacts with dietary fatty acids intake to modulate inflammation in type 2 diabetes mellitus patients
Predictive Values of Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin for Delayed Graft Function Diagnosis in Kidney Transplantation
Effect of Hypertonic Saline 5% on Early Graft Function and Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin in Deceased-Donor Kidney Transplantation
Caspase-1 and interleukin-18 in children with post infectious bronchiolitis obliterans: a case-control study
Interleukin-18 serum level before and after intralesional immunotherapy with tuberculin purified protein derivative in patients with cutaneous and genital warts
View all publications for the product

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 96T

    E0147Hu

    $458.00

  • 48T

    E0147Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up