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Home > Product > ELISA Kit > Human E-Cadherin, E-CAD ELISA Kit

Human E-Cadherin, E-CAD ELISA Kit

Human E-Cadherin, E-CAD ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.52ng/ml

Detection range: 1-300ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.52ng/ml

Detection range: 1-300ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

E-Cadherin

Code

E0209Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.52ng/ml

Detection range

1-300ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cadherins are calcium-dependent cell adhesion proteins (PubMed: 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed: 11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

UniProt accession

MASS(Da)

97,456

GeneID

999

Synonyms

Cadherin-1;CAM 120/80;CD antigen CD324;CDH 1;CDH1;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;E-cadherin;Epithelial cadherin;Uvomorulin

Gene names

CDH1

Research area

Cancer

Target protein

CDH1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0209Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
200ng/ml 2.065 2.117 2.076
2.169
100ng/ml 1.499 1.401 1.359
1.302
50ng/ml 0.78 0.734 0.693
0.688
25ng/ml 0.322 0.359 0.318
0.396
12.5ng/ml 0.187 0.152 0.111
0.118
0ng/ml 0.032 0.041 0
0.05

Precision

We measured random samples of E0209Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 99.7 3.72 3.7
Intra-Assay 2 18 57.5 2.48 4.3
Intra-Assay 3 18 47.9 1.8 3.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0209Hu

    $458.00

  • 48T

    E0209Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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