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Home > Product > ELISA Kit > Rat Interleukin 10, IL-10 ELISA Kit

Rat Interleukin 10, IL-10 ELISA Kit

Rat Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Rat

Product Summary

Size: 96T,48T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Rat

Product overview

Full product name

Interleukin 10

Code

E0108Ra

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.51pg/ml

Detection range

3-900pg/ml

Sample type

Serum, plasma, cell culture supernates, ascites, tissue homogenates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. 

UniProt accession

MASS(Da)

20,426

GeneID

25325

Synonyms

Interleukin-10; IL-10; IL 10; IL10; Cytokine synthesis inhibitory factor; CSIF

Gene names

IL10

Research area

Immunology

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Interleukin 10, IL-10 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480pg/ml 2.165 2.154 2.096
2.144
240pg/ml 1.769 1.729 1.671
1.689
120pg/ml 1.105 1.15 1.092
1.194
60pg/ml 0.634 0.629 0.571
0.624
30pg/ml 0.431 0.422 0.365
0.414
0pg/ml 0.052 0.058 0
0.064

Precision

We measured random samples of E0108Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 44.4 2.39 5.4
Intra-Assay 2 18 122.9 8.15 6.6
Intra-Assay 3 18 140.8 5.5 3.9

Protocols

References

The Efficacy of Human Dental Pulp Stem Cells in regenerating Submandibular Gland Defects in Diabetic Wistar Rats (Rattus novergicus)

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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