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Home > Product > ELISA Kit > Rat Interleukin 10, IL-10 ELISA Kit

Rat Interleukin 10, IL-10 ELISA Kit

Rat Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Interleukin 10

Code

E0108Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.51pg/ml

Detection range

3-900pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

MASS(Da)

20426

GeneID

25325

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0108Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480pg/ml 2.165 2.154 2.096
2.144
240pg/ml 1.769 1.729 1.671
1.689
120pg/ml 1.105 1.15 1.092
1.194
60pg/ml 0.634 0.629 0.571
0.624
30pg/ml 0.431 0.422 0.365
0.414
0pg/ml 0.052 0.058 0
0.064

Precision

We measured random samples of E0108Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 44.4 2.39 5.4
Intra-Assay 2 18 122.9 8.15 6.6
Intra-Assay 3 18 140.8 5.5 3.9

Protocols

References (12)

Evaluation of anti-amnesic effect of Conyza bonariensis in rats
Synergistic Effect of Omega-3 and Probiotic Supplementation on Preventing Ligature-Induced Periodontitis
The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats
Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level
Anti-Inflammatory Effect of Crude Momordica charantia L. Extract on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Model in Rat and the Bioaccessibility of its Carotenoid Content
View all publications for the product

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  • Size

    Code

    Price

  • 48T

    E0108Ra

    $320.00

  • 96T

    E0108Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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