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Home > Product > ELISA Kit > Rat Tumor Necrosis Factor Α, TNF-A ELISA Kit

Rat Tumor Necrosis Factor Α, TNF-A ELISA Kit

Rat Tumor Necrosis Factor Α, TNF-A ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 96T,48T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Tumor Necrosis Factor Α

Code

E0764Ra

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.51ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

UniProt accession

MASS(Da)

25,806

GeneID

10369438024835

Synonyms

APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; tnf alpha; tnf a; TNFSF2; Tumor necrosis factor; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form

Gene names

TNFA

Research area

Immunology

Target protein

TNFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Tumor Necrosis Factor Α, TNF-A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.106 2.175 2.113
2.243
320ng/L 1.464 1.333 1.272
1.202
160ng/L 0.787 0.845 0.784
0.903
80ng/L 0.472 0.489 0.428
0.506
40ng/L 0.269 0.265 0.204
0.261
0ng/L 0.078 0.061 0
0.045

Precision

We measured random samples of E0764Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 247.3 13.39 5.4
Intra-Assay 2 18 426.1 19.65 4.6
Intra-Assay 3 18 114 6.51 5.7

Protocols

References

Evaluation of the effects of the hydroalcoholic extract of Terminalia chebulafruits on diazinon-induced liver toxicity and oxidative stress in rats
Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats
Increased Serum Levels of Tumor Necrosis Factor-Alpha, Resistin, and Visfatin in the Children with Autism Spectrum Disorders: A Case-Control Study
Evaluation of the Effect of Ascorbic Acid Administration on Gene Expression Level of IL-6 and TNF-α Cytokines in Deceased Donors.
Protective and anti-inflammatory effects of silymarin on paraquat-induced nephrotoxicity in rats
Anti-Rheumatoid activity of Sesamin Complex in Freund's Complete Adjuvant induced arthritis in Wistar albino rats
Acetate ameliorates nephrotoxicity in streptozotocin-nicotinamide-induced diabetic rats: Involvement of xanthine oxidase activity
Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
Effect of egg white and honey enema on ulcerative colitis in an animal model

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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