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Home > Product > ELISA Kit > Human Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Human Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Human Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.25ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.25ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytotoxic T Lymphocyte Associated Antigen 4

Code

E0277Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.25ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

UniProt accession

MASS(Da)

24,656

GeneID

1493

Synonyms

CD antigen CD152;CTLA 4;CTLA4;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Gene names

CTLA4

Research area

Immunology

Target protein

CTLA4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0277Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.071 2.232 2.189
2.393
40ng/ml 1.137 1.248 1.205
1.36
20ng/ml 0.797 0.757 0.714
0.717
10ng/ml 0.52 0.456 0.413
0.392
5ng/ml 0.179 0.192 0.149
0.206
0ng/ml 0.033 0.043 0
0.054

Precision

We measured random samples of E0277Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 44.8 1.37 3
Intra-Assay 2 18 31.4 1.61 5.1
Intra-Assay 3 18 9.4 0.67 7.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0277Hu

    $458.00

  • 48T

    E0277Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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