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Home > Product > ELISA Kit > Rat Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Rat Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Rat Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.08ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.08ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Acetyl-Coa Carboxylase 1

Code

E1839Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.08ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

UniProt accession

MASS(Da)

265,194

GeneID

60581

Synonyms

ACACA;ACC1;ACC-alpha;Acetyl-CoA carboxylase 1

Gene names

ACACA

Research area

Others

Target protein

ACACA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1839Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.65 2.669 2.627
2.687
800ng/L 1.443 1.503 1.461
1.562
400ng/L 0.941 0.91 0.869
0.879
200ng/L 0.455 0.456 0.414
0.456
100ng/L 0.216 0.242 0.201
0.268
0ng/L 0.035 0.041 0
0.047

Precision

We measured random samples of E1839Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 584.2 34.95 6
Intra-Assay 2 18 885.9 24.47 2.8
Intra-Assay 3 18 227.7 12.61 5.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1839Ra

    $320.00

  • 96T

    E1839Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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