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Home > Product > ELISA Kit > Bovine Interleukin 18, IL-18 ELISA Kit

Bovine Interleukin 18, IL-18 ELISA Kit

Bovine Interleukin 18, IL-18 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 9.99ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 9.99ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Interleukin 18

Code

E0318Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

9.99ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.

UniProt accession

MASS(Da)

22,347

GeneID

281249

Synonyms

IFN-gamma-inducing factor;IL 18;IL-1 gamma;IL18;IL-18;Interferon gamma-inducing factor;Interleukin-1 gamma;Interleukin-18

Gene names

IL18

Research area

Immunology

Target protein

IL18

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Bovine Interleukin 18, IL-18 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.274 2.251 2.199
2.229
1200ng/L 1.726 1.776 1.724
1.827
600ng/L 1.204 1.255 1.202
1.305
300ng/L 0.688 0.69 0.637
0.692
150ng/L 0.393 0.413 0.36
0.433
0ng/L 0.064 0.052 0
0.041

Precision

We measured random samples of E0318Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 322.1 20.16 6.3
Intra-Assay 2 18 1270.8 69.63 5.5
Intra-Assay 3 18 202.8 10.4 5.1

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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