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Home > Product > ELISA Kit > Bovine Interleukin 4, IL-4 ELISA Kit

Bovine Interleukin 4, IL-4 ELISA Kit

Bovine Interleukin 4, IL-4 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 3.34ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 3.34ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Interleukin 4

Code

E0036Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

3.34ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

UniProt accession

MASS(Da)

15,119

GeneID

280824

Synonyms

B-cell stimulatory factor 1;BSF-1;IL 4;IL4;IL-4;Interleukin-4;Lymphocyte stimulatory factor 1

Gene names

IL4

Research area

Immunology; Cancer

Target protein

IL4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0036Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.612 2.564 2.531
2.516
400ng/L 1.503 1.416 1.383
1.328
200ng/L 0.948 0.889 0.856
0.831
100ng/L 0.48 0.497 0.464
0.514
50ng/L 0.196 0.204 0.171
0.211
0ng/L 0.031 0.033 0
0.035

Precision

We measured random samples of E0036Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 395.6 12.83 3.2
Intra-Assay 2 18 134.7 7.5 5.6
Intra-Assay 3 18 255.4 13.2 5.2

Protocols

References (2)

High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi
Indoleamine 2, 3-dioxygenase 1 mediated alterations in the functionality of immune cells, deciphers the pregnancy outcomes in crossbred dairy cows

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  • Size

    Code

    Price

  • 96T

    E0036Bo

    $458.00

  • 48T

    E0036Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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