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Home > Product > ELISA Kit > Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase, IFI30 ELISA Kit

Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase, IFI30 ELISA Kit

Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase, IFI30 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Gamma-Interferon-Inducible Lysosomal Thiol Reductase

Code

E0329Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.01ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).

UniProt accession

MASS(Da)

27,490

GeneID

615930

Synonyms

Gamma-interferon-inducible lysosomal thiol reductase;IFI 30;IFI30;IFI30 protein

Gene names

IFI30

Research area

Immunology

Target protein

IFI30

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase, IFI30 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.141 2.069 2.018
1.997
4ng/ml 0.932 0.939 0.887
0.945
2ng/ml 0.491 0.482 0.431
0.473
1ng/ml 0.319 0.247 0.195
0.175
0.5ng/ml 0.164 0.147 0.096
0.13
0ng/ml 0.054 0.051 0
0.048

Precision

We measured random samples of E0329Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.2 0.14 4.4
Intra-Assay 2 18 5.5 0.12 2.2
Intra-Assay 3 18 2.9 0.13 4.7

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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