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Home > Product > ELISA Kit > Human Semaphorin 4D, SEMA4D ELISA Kit

Human Semaphorin 4D, SEMA4D ELISA Kit

Human Semaphorin 4D, SEMA4D ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.1ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.1ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Semaphorin 4D

Code

E1566Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.1ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed: 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed: 19788569). Promotes the migration of cerebellar granule cells (PubMed: 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed: 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed: 16055703).

UniProt accession

MASS(Da)

96,150

GeneID

10507

Synonyms

A8;BB18;CD antigen CD100;GR3;SEMA4D;Semaphorin-4D

Gene names

SEMA4D

Research area

Neuroscience

Target protein

SEMA4D

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1566Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.082 2.102 2.053
2.122
16ng/ml 1.33 1.387 1.338
1.444
8ng/ml 0.82 0.822 0.773
0.824
4ng/ml 0.49 0.463 0.414
0.436
2ng/ml 0.292 0.273 0.224
0.254
0ng/ml 0.046 0.049 0
0.052

Precision

We measured random samples of E1566Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.8 0.21 5.5
Intra-Assay 2 18 17.4 0.72 4.2
Intra-Assay 3 18 12.5 0.87 7

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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