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Home > Product > ELISA Kit > Human T-lymphocyte Activation Antigen Cd86, CD86 ELISA Kit

Human T-lymphocyte Activation Antigen Cd86, CD86 ELISA Kit

Human T-lymphocyte Activation Antigen Cd86, CD86 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

T-Lymphocyte Activation Antigen CD86

Code

E1572Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-40.ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed: 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). [Isoform 2]: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

UniProt accession

MASS(Da)

37,682

GeneID

942

Synonyms

Activation B7-2 antigen;B70;BU63;CD 86;CD antigen CD86;CD86;CTLA-4 counter-receptor B7.2;FUN-1;T-lymphocyte activation antigen CD86;

Gene names

CD86

Research area

Immunology

Target protein

CD86

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1572Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.323 2.225 2.183
2.126
12ng/ml 1.328 1.297 1.255
1.265
6ng/ml 0.617 0.67 0.628
0.723
3ng/ml 0.409 0.355 0.314
0.302
1.5ng/ml 0.138 0.163 0.121
0.188
0ng/ml 0.038 0.042 0
0.045

Precision

We measured random samples of E1572Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.2 0.17 5.1
Intra-Assay 2 18 12.2 0.3 2.4
Intra-Assay 3 18 6.7 0.3 4.4

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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