Human Fibronectin, FN ELISA Kit

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Home > Product > ELISA Kit > Human Fibronectin, FN ELISA Kit

Human Fibronectin, FN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.02ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.02ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fibronectin

Code

E2002Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.02ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed: 3024962, PubMed: 3900070, PubMed: 3593230, PubMed: 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed: 3024962, PubMed: 3900070, PubMed: 3593230, PubMed: 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

UniProt accession

P02751

MASS（Da）

272,320

GeneID

2335

Synonyms

Anastellin;CIG;Cold-insoluble globulin;Fibronectin;FN;FN 1;FN1;Ugl-Y1;Ugl-Y2;Ugl-Y3

Gene names

FN1

Research area

Cardiovascular

Target protein

FN1

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E2002Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/ml	1.95	2.119	2.08
	2.288
200ng/ml	1.542	1.428	1.389
	1.313
100ng/ml	0.688	0.633	0.594
	0.577
50ng/ml	0.398	0.375	0.336
	0.352
25ng/ml	0.179	0.183	0.144
	0.187
0ng/ml	0.027	0.039	0
	0.051

Precision

We measured random samples of E2002Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	215.4	6.27	2.9
Intra-Assay	2	18	69.3	3.3	4.8
Intra-Assay	3	18	127	6.06	4.8

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Associated products

Datasheet

Size

Code

Price

48T

E2002Hu

$320.00
96T

E2002Hu

$458.00

Add to Cart

Lead time: Within two weeks

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