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Home > Product > ELISA Kit > Human Caspase 14, CASP14 ELISA Kit

Human Caspase 14, CASP14 ELISA Kit

Human Caspase 14, CASP14 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.33ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.33ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Caspase 14

Code

E2252Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.33ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed: 15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed: 16854378, PubMed: 19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed: 25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed: 24743736).

UniProt accession

MASS(Da)

27,680

GeneID

23581

Synonyms

CASP 14;CASP14;CASP-14;Caspase-14;Caspase-14 subunit p10, mature form;Caspase-14 subunit p17, mature form;Caspase-14 subunit p20, intermediate form;Caspase-14 subunit p8, intermediate form

Gene names

CASP14

Research area

Others

Target protein

CASP14

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2252Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.071 2.05 2.001
2.028
2000ng/L 1.374 1.36 1.312
1.347
1000ng/L 0.542 0.616 0.567
0.691
500ng/L 0.408 0.429 0.38
0.449
250ng/L 0.187 0.181 0.132
0.174
0ng/L 0.054 0.049 0
0.043

Precision

We measured random samples of E2252Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2016.7 62.85 3.1
Intra-Assay 2 18 1225.2 60.95 5
Intra-Assay 3 18 683.1 30.44 4.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2252Hu

    $320.00

  • 96T

    E2252Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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