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Home > Product > ELISA Kit > Human Diacylglycerol-O-Acyltransferase Homolog 1, DGAT1 ELISA Kit

Human Diacylglycerol-O-Acyltransferase Homolog 1, DGAT1 ELISA Kit

Human Diacylglycerol-O-Acyltransferase Homolog 1, DGAT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.66ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.66ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Diacylglycerol-O-Acyltransferase Homolog 1

Code

E2652Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.66ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed: 16214399, PubMed: 18768481, PubMed: 28420705, PubMed: 9756920, PubMed: 32433611, PubMed: 32433610). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed: 18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed: 16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed: 18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed: 16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester Synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed: 28420705).

UniProt accession

MASS(Da)

55,278

GeneID

8694

Synonyms

ACAT-related gene product 1;Acyl-CoA retinol O-fatty-acyltransferase;ARAT;DGAT 1;DGAT1;Diacylglycerol O-acyltransferase 1;Diglyceride acyltransferase;Retinol O-fatty-acyltransferase

Gene names

DGAT1

Research area

Others

Target protein

DGAT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2652Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.062 2.198 2.14
2.334
600ng/L 1.559 1.461 1.403
1.363
300ng/L 0.956 0.877 0.819
0.798
150ng/L 0.402 0.469 0.411
0.535
75ng/L 0.263 0.271 0.213
0.279
0ng/L 0.063 0.058 0
0.053

Precision

We measured random samples of E2652Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 640.2 23.33 3.6
Intra-Assay 2 18 362.5 20.17 5.6
Intra-Assay 3 18 174 9 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2652Hu

    $320.00

  • 96T

    E2652Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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