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Home > Product > ELISA Kit > Bovine Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Bovine Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Bovine Glutamate Dehydrogenase 1, Mitochondrial, GLUD1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.55mU/mL

Detection range: 1-380mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.55mU/mL

Detection range: 1-380mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Glutamate Dehydrogenase 1, Mitochondrial

Code

E2211Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.55mU/mL

Detection range

1-380mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed: 4365183, PubMed: 14659072). Plays a role in insulin homeostasis (By similarity). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

UniProt accession

MASS(Da)

61,512

GeneID

281785

Synonyms

GDH 1;GLUD 1;GLUD1;Glutamate dehydrogenase 1, mitochondrial

Gene names

GLUD1

Research area

Others

Target protein

GLUD1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2211Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
200mU/mL 2.039 2.041 2.004
2.042
100mU/mL 1.519 1.559 1.523
1.598
50mU/mL 1.083 1.021 0.985
0.959
25mU/mL 0.651 0.686 0.65
0.72
12.5mU/mL 0.404 0.423 0.387
0.442
0mU/mL 0.034 0.036 0
0.038

Precision

We measured random samples of E2211Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 96.6 4.97 5.1
Intra-Assay 2 18 45.3 3.11 6.9
Intra-Assay 3 18 10.3 0.76 7.4

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

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