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Home > Product > ELISA Kit > Human Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Human Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Human Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Angiotensin Converting Enzyme 2

Code

E3169Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed: 27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid Peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed: 10969042, PubMed: 10924499, PubMed: 11815627, PubMed: 19021774, PubMed: 14504186). Also removes the C-terminal residue from three other vasoactive Peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed: 10969042, PubMed: 11815627). Also cleaves other biological Peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed: 11815627, PubMed: 27217402, PubMed: 28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed: 18424768, PubMed: 19185582). [Isoform 2]: Non-functional as a carboxypeptidase. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. [Isoform 2]: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.

UniProt accession

MASS(Da)

92,463

GeneID

59272

Synonyms

ACE 2;ACE2;ACEH;ACE-related carboxypeptidase;Angiotensin-converting enzyme 2;Angiotensin-converting enzyme homolog;Angiotensin-converting enzyme-related carboxypeptidase;Metalloprotease MPROT15;Processed angiotensin-converting enzyme 2;

Gene names

ACE2

Research area

Microbiology; Cell Biology; Cardiovascular

Target protein

ACE2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3169Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.029 2.153 2.103
2.277
6ng/ml 1.786 1.778 1.728
1.771
3ng/ml 1.095 1.193 1.143
1.291
1.5ng/ml 0.636 0.667 0.617
0.697
0.75ng/ml 0.402 0.414 0.364
0.426
0ng/ml 0.067 0.05 0
0.033

Precision

We measured random samples of E3169Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.4 0.18 5.3
Intra-Assay 2 18 1.9 0.1 5.3
Intra-Assay 3 18 1.6 0.08 4.8

Protocols

References (4)

HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism
Assessment of the levels of some prognostic biomolecules (galectins, ACE2, SCUBE1/2/3) in COVID-19 patients
Assessment of Liver Regeneration in Patients Who Have Undergone Living Donor Hepatectomy for Living Donor Liver Transplantation
Design, DFT and molecular doping studies of metal complexes as a neurotransmitter modulator of autism spectrum disease in preschool children

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  • Size

    Code

    Price

  • 48T

    E3169Hu

    $320.00

  • 96T

    E3169Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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