Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Acetyl-Coa Carboxylase 1

Code

E3172Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.34ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed: 20952656, PubMed: 20457939, PubMed: 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed: 20952656, PubMed: 20457939, PubMed: 29899443).

UniProt accession

MASS(Da)

265,554

GeneID

31

Synonyms

ACACA;ACC1;ACC-alpha;Acetyl-CoA carboxylase 1;Acetyl-Coenzyme A carboxylase alpha

Gene names

ACACA

Research area

Metabolism

Target protein

ACACA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3172Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.071 2.103 2.064
2.135
320ng/L 1.598 1.618 1.579
1.638
160ng/L 1.076 1.098 1.059
1.12
80ng/L 0.708 0.745 0.706
0.783
40ng/L 0.452 0.433 0.394
0.414
0ng/L 0.036 0.039 0
0.042

Precision

We measured random samples of E3172Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 415.7 27.8 6.7
Intra-Assay 2 18 114.2 5.19 4.5
Intra-Assay 3 18 35 2.01 5.8

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E3172Hu

    $320.00

  • 96T

    E3172Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up