Product overview
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Background
IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
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MASS（Da）
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0119Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.03 | 2.053 | 1.995 |
| 2.076 | |||
| 20ng/ml | 1.56 | 1.576 | 1.518 |
| 1.592 | |||
| 10ng/ml | 1.004 | 0.945 | 0.887 |
| 0.887 | |||
| 5ng/ml | 0.479 | 0.474 | 0.416 |
| 0.47 | |||
| 2.5ng/ml | 0.253 | 0.259 | 0.2 |
| 0.264 | |||
| 0ng/ml | 0.06 | 0.0581 | 0 |
| 0.056 |
Precision
We measured random samples of E0119Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.8 | 0.39 | 4.4 |
| Intra-Assay | 2 | 18 | 17.5 | 0.75 | 4.3 |
| Intra-Assay | 3 | 18 | 5.5 | 0.26 | 4.8 |
Protocols
References (12)
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