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Home > Product > ELISA Primacu > Human Ferritin Light Chain, FT Primacu™ ELISA Kit

Human Ferritin Light Chain, FT Primacu™ ELISA Kit

Human Ferritin Light Chain, FT Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 9.81 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 9.81 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ferritin light chain

Code

BPE255

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

9.81 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.

UniProt accession

MASS(Da)

20020

GeneID

2512

Gene names

FTL

Research area

Cardiovascular,Metabolism,Neuroscience,Signal transduction

Target protein

Ferritin light chain

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE255 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 2.311 2.395 2.27
2.478
1500 pg/ml 1.469 1.421 1.296
1.372
750 pg/ml 0.765 0.779 0.654
0.792
375 pg/ml 0.492 0.461 0.336
0.43
187.5 pg/ml 0.3 0.29 0.165
0.28
93.75 pg/ml 0.192 0.2 0.075
0.207
46.875 pg/ml 0.177 0.176 0.051
0.175
0 pg/ml 0.133 0.125 0
0.117

Precision

We measured random samples of BPE255 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 389 36.1 9.3
Intra-assay 2 20 972 88.6 9.1
Intra-assay 3 20 2261 108 4.8
Inter-assay 1 20 386 37.3 9.7
Inter-assay 2 20 967 81.5 8.4
Inter-assay 3 20 2237 164 7.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE255

    $2320.00

  • 96T

    BPE255

    $580.00

  • 15*96T

    BPE255

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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