Human Thyroid Peroxidase, TPO Primacu™ ELISA Kit

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Coagulation sanguine
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Home > Product > ELISA Primacu > Human Thyroid Peroxidase, TPO Primacu™ ELISA Kit

Human Thyroid Peroxidase, TPO Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.85 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.85 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Thyroid peroxidase

Code

BPE268

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

10.85 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.

UniProt accession

P07202

MASS（Da）

102963

GeneID

7173

Gene names

TPO

Research area

Cancer,Metabolism,Signal transduction,Neuroscience

Target protein

Thyroid peroxidase

Components

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

Protocol data

These standard curves of BPE268 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1500 pg/ml	2.421	2.495	2.376
	2.568
750 pg/ml	1.479	1.403	1.284
	1.326
375 pg/ml	0.801	0.8	0.681
	0.798
187.5 pg/ml	0.449	0.475	0.356
	0.5
93.75 pg/ml	0.298	0.296	0.177
	0.293
46.875 pg/ml	0.214	0.204	0.085
	0.193
23.4375 pg/ml	0.177	0.168	0.049
	0.159
0 pg/ml	0.126	0.119	0
	0.112

Precision

We measured random samples of BPE268 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	130	5.9	4.5
Intra-assay	2	20	272	14.2	5.2
Intra-assay	3	20	877	54	6.2
Inter-assay	1	20	130	8.1	6.2
Inter-assay	2	20	274	14	5.1
Inter-assay	3	20	886	43.1	4.9

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

5*96T

BPE268

$2040.00
96T

BPE268

$480.00
15*96T

BPE268

$5400.00

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Lead time: Within two weeks

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