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Home > Product > ELISA Primacu > Human Carbonic Anhydrase 12, CA12 Primacu™ ELISA Kit

Human Carbonic Anhydrase 12, CA12 Primacu™ ELISA Kit

Human Carbonic Anhydrase 12, CA12 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.49 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.49 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carbonic anhydrase 12

Code

BPE113

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.49 pg/mL

Detection range

7.81-500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

UniProt accession

MASS(Da)

39451

GeneID

771

Gene names

CA12

Research area

Cancer,Neuroscience

Target protein

carbonic anhydrase XII

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE113 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
500 pg/ml 2.012 2.077 1.947
2.142
250 pg/ml 1.096 1.084 0.954
1.072
125 pg/ml 0.595 0.597 0.467
0.599
62.5 pg/ml 0.356 0.378 0.248
0.401
31.25 pg/ml 0.249 0.254 0.124
0.259
15.625 pg/ml 0.178 0.188 0.058
0.199
7.8125 pg/ml 0.155 0.16 0.03
0.165
0 pg/ml 0.136 0.13 0
0.124

Precision

We measured random samples of BPE113 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 62.5 1.6 2.6
Intra-assay 2 20 145 4.8 3.3
Intra-assay 3 20 346 11.6 3.4
Inter-assay 1 20 63.1 5.2 8.3
Inter-assay 2 20 144 9.8 6.8
Inter-assay 3 20 348 14.9 4.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE113

    $2320.00

  • 96T

    BPE113

    $580.00

  • 15*96T

    BPE113

    $6525.00

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Lead time: Within two weeks

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