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Home > Product > ELISA Kit > Porcine Rho-associated Protein Kinase 2, ROCK2 ELISA Kit

Porcine Rho-associated Protein Kinase 2, ROCK2 ELISA Kit

Porcine Rho-associated Protein Kinase 2, ROCK2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Rho-associated protein kinase 2

Code

E0499Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/ml

Detection range

0.1-35ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

UniProt accession

MASS(Da)

160,750

GeneID

397445

Synonyms

p164 ROCK-2;Rho kinase 2;Rho-associated protein kinase 2;Rho-associated, coiled-coil-containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;ROCK2;ROCK-II

Gene names

ROCK2

Research area

Cell Biology; Signal Transduction; Cancer

Target protein

ROCK2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0499Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.112 2.087 2.038
2.063
10ng/ml 1.338 1.353 1.303
1.367
5ng/ml 0.933 0.89 0.841
0.847
2.5ng/ml 0.484 0.443 0.393
0.402
1.25ng/ml 0.265 0.268 0.218
0.27
0ng/ml 0.063 0.05 0
0.036

Precision

We measured random samples of E0499Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.19 0.28 6.73
Intra-Assay 2 18 10.82 0.49 4.52
Intra-Assay 3 18 5.96 0.36 5.96

Protocols

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  • Size

    Code

    Price

  • 48T

    E0499Po

    $320.00

  • 96T

    E0499Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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