25-Hydroxyvitamin D-1 Alpha Hydroxylase,Mitochondrial

E3868Hu

Sandwich

48T,96T

9.47ng/L

20-3800ng/L

Serum, plasma, cell culture supernates

Human

2-8ºC

1h 30m

A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed: 10518789, PubMed: 9486994, PubMed: 22862690, PubMed: 10566658, PubMed: 12050193). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed: 10518789, PubMed: 22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed: 10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed: 22862690).

56,504

1594

25-hydroxyvitamin D(3 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-OHD-1 alpha-hydroxylase;Calcidiol 1-monooxygenase;CYP27B1;Cytochrome p450 27B1;Cytochrome P450 subfamily XXVIIB polypeptide 1;Cytochrome P450C1 alpha;Cytochrome P450VD1-alpha;VD3 1A hydroxylase

CYP27B1

Others

CYP27B1