Product name
Recombinant Rat MDC/CCL22 protein
Code
CD00091
Biological activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rRtMDC/CCL22 as determined by LAL method.
Synonyms
MDC/CCL22, Mdc, Scya22,Rat
Sequence
GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA
Uniprot Accession
Molecular Weight
Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL22 is a protein encoded by the CCL22 gene. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).
Storage
-20ºC
Research area
Immunology
