Product name
Recombinant Rat IL-7 protein
Code
CD00100
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtIL-7 as determined by LAL method.
Synonyms
IL-7, IL7,Rat
Sequence
DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI
Uniprot Accession
Molecular Weight
Approximately 14. 9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and Mouse IL-7.
Storage
-20ºC
Research area
Immunology
